报告题目：Bioproduction in the algal chloroplast
报告人：Prof. Saul Purton
Department of Structural & Molecular Biology, University College London
报告摘要：Microalgae have significant potential as light-driven cell factories for the synthesis of a wide range of bioproducts. These include bioactive metabolites, high-value pigments, speciality enzymes, therapeutic proteins, biopolymers and RNA-based anti-viral agents. The genetic engineering of algae is most advanced for the model species Chlamydomonas reinhardtii, with well-established methods for manipulating both the nuclear and chloroplast genomes. The latter is particularly attractive as a site for insertion of foreign genes for a number of reasons: i) genes can be targeted to precise loci; ii) stable, high level expression can be achieved; iii) the small, low complexity genome is particularly suited to synthetic biology (synbio) approaches that allow rapid, standardised and predictive strain engineering; iv) the organelle is the site of numerous biosynthetic pathways and therefore represents the obvious ‘chassis’ on which to bolt new metabolic pathways that divert the carbon fixed by photosynthesis into novel compounds. I will present our recent progress on the development of synbio tools for genetic engineering of the chloroplast of C. reinhardtii, including a simple ‘one-pot’ method for DNA assembly and selection methods that avoid the use of antibiotic-based markers. Our tools also include design strategies for biocontainment of transgenes, and their high-level and inducible expression, together with a method for low-cost algal cultivation in non-sterilised media. I will give examples of applications in the field of therapeutic proteins including the production of anti-bacterial enzymes that target major human pathogens, and bio-encapsulated vaccines for oral delivery in the aquaculture industry.