学习经历
2005-2009 河北农业大学,学士
2009-2012 中国农业科学院,硕士
2012-2017 中国科学院,博士
工作经历
2017-2019 首都师范大学生命科学学院,博士后
2019- 河北农业大学生命科学学院,教授
荣誉称号及社会兼职
太行学者二层次人才,河北省优秀青年基金获得者,河北省教育厅青年拔尖人才
aBIOTECH期刊青年编委
教学工作(本科生、研究生)
本科生课程:
1. 专业基础课《植物生理学》
2. 专业选修课《生态学》
研究生课程:
1.《现代生物技术与方法》
2.《高级发育生物学》
3.《高级植物生理学》
主持课题项目
1.国家自然科学基金,基于CRISPR/Cas9技术解析γ-氨基丁酸调控大白菜耐热性的分子机制,2025-2027,32402564,主持
2.河北省自然科学基金,高效腺嘌呤碱基编辑体系的建立及其在创制富含萝卜硫苷大白菜中的应用研究,2023-2025,C2023204119,主持
3.河北省省级科技计划专项,植物基因组无PAM序列限制的CRISPR/Cas9编辑体系的建立及应用,2021-2023,21372901D,主持
4.河北省教育厅青年拔尖人才计划项目,MAC3A和MAC3B调控拟南芥雄配子体发育的研究,2021-2023,BJ2021025,主持
5.河北省优秀青年科学基金,组蛋白H2B单泛素化修饰参与小立碗藓环境适应的机制研究,2020-2022,C2020204062,主持
6.河北农业大学人才引进项目,小立碗藓H2B单泛素化的研究,2019-2024,YJ201958,主持
代表性学术成果
研究论文(*通讯作者):
1.Shan Q1, Wang Y1, Li J1, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi J, Qiu J, Gao C* (2013) Targeted genome modification of crop plants using a CRISPR-Cas system. Nature Biotechnology, 31, 686-688.(共同第一作者,中科院一区top)
2.Li J, Meng X, Zong Y, Chen K, Zhang H, Liu J, Li J*, Gao C* (2016) Gene replacements and insertions in rice by intron targeting using CRISPR/Cas9. Nature Plants, 2, 16139.(第一作者,中科院一区top)
3.Li J, Zhang H, Si X, Tian Y, Chen K, Liu J, Chen H, Gao C* (2017) Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene. Journal of Genetics and Genomics, 44, 465-468.(第一作者,中科院二区)
4.Li J, Li Y, Ma L* (2019) CRISPR/Cas9-based genome editing and its applications for functional genomic analyses in plants. Small Methods, 3, 1800473.(第一作者,中科院一区)
5.Li J, Yang J, Li Y, Ma L* (2020) Current strategies and advances in wheat biology. The Crop Journal, 8, 879-891.(第一作者,中科院一区)
6.Li J, Li Y, Ma L* (2021) Recent advances in CRISPR/Cas9 and applications for wheat functional genomics and breeding. aBIOTECH, 3, 1800473.(第一作者,中科院四区)
7.Li Y, Li WJ, Li J* (2021) The CRISPR/Cas9 revolution continues: from base editing to prime editing in plant science. Journal of Genetics and Genomics, 48, 661-670.(通讯作者,中科院二区)
8.Li J*, Yu X, Zhang C, Li N, Zhao J* (2022) The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives. aBIOTECH, 3, 146–161.(第一兼通讯作者,中科院四区)
9.Zhang C, Wang D, Li W, Zhang B, Abdel-Fattah Ouf GM, Su X*, Li J* (2022) The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids. Frontiers in Microbiology, 13,951479. (通讯作者,中科院二区)
10.Zhang C, Liu S, Li X, Zhang R, Li J* (2022) Virus-Induced Gene Editing and Its Applications in Plants. International Journal of Molecular Sciences. 23, 10202.(通讯作者,中科院二区)
11.Zhang K, Liu S, Fu Y, Wang Z, Yang X, Li W, Zhang C, Zhang D, Li J* (2023) Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis. Plant Methods. 19, 5.(通讯作者,中科院二区)
12.Yu X, Huo G, Yu J, Li H, Li J* (2023) Prime editing: Its systematic optimization and current applications in disease treatment and agricultural breeding. International Journal of Biological Macromolecules. 253, 127025.(通讯作者,中科院一区)
13.Li W, Li X, Wang C, Huo G, Zhang X, Yu J, Yu X, Li J, Zhao J, Li Y, Li J* (2024) Expanding the targeting scope of CRISPR/Cas9-mediated genome editing by Cas9 variants in Brassica. aBIOTECH, 5, 202–208.
14.Yu X, Yu J, Lu Y, Li W, Huo G, Zhang J, Li Y, Zhao J, Li J* (2024) An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops. Horticultural Plant Journal, 10, 983-994.(通讯作者,中科院一区)
15.李君,张毅,陈坤玲,单奇伟,王延鹏,梁振,高彩霞*. CRISPR/Cas系统:RNA靶向的基因组定向编辑新技术. 遗传, 2013, 35, 1265-1273. (第一作者)
16.刘善贺, 潘延云, 李君* (2023) 组蛋白H2B单泛素化在植物生长发育和胁迫应答中的作用. 农业生物技术学报. 31, 1064-1073.(通讯作者)